Immunity Response of Chickens (Gallus Gallus Domesticus) in the Early Growth Phase Post Injection Lipopolysaccharide (Lps) E.Coli Atcc 25922
DOI:
https://doi.org/10.19184/bioedu.v23i2.53737Keywords:
Immune system, LPS E.coli, Native Chicken, Blood SmearAbstract
Local Indonesian chickens are an important source of animal protein, especially in small-scale farming. However, these farms often face challenges with poor hygiene, which can lead to infections. A common bacterial culprit is E.coli, a Gram-negative bacterium whose outer membrane contains lipopolysaccharide (LPS). As an endotoxin, LPS triggers an immune response in the host, leading to inflammation and fever.. This study investigated the effect of LPS injection on the body temperature and leukocyte differentiation as an immuno-competence respond of native chickens. The LPS solution was extracted using a hot phenol- water method and then injected into the abdominal cavities of chickens at 14, 28, and 32 days of age. Body temperature was measured 12 hours after each injection. The immunological respond of the chicks was investigated via leukocyte differentiation using bloodsmear analysis. The results showed a significant increase in body temperature in LPS-injected chickens compared to the control group. This difference persisted across all three injections, indicating that LPS effectively stimulates the immune system. Additional support for immune stimulation came from the leukocyte differential, which revealed a notable difference in Basophil percentage in the treated chicks. Interestingly, repeated LPS stimulation also led to significant changes in overall leukocyte differentiation. These findings collectively demonstrate that LPS injection successfully enhances the immuno-competence of chicks. We realize the unique character of the immunocompetence chicks could play a role as an important in avian immunty which warrant to study in future.
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